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V5 tag Monoclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB

Reactivity

  • Species independent
Overview
Catalog # bsm-33005m-bf555
Product Name V5 tag Monoclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB
Reactivity Species independent
Specifications
Conjugation AbBy Fluor-555
Host Mouse
Source KLH conjugated V5-tag peptide
Clonality Monoclonal
Clone # 5D7
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag.
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is V5 tag Monoclonal Antibody, AbBy Fluor-555 Conjugated (bsm-33005M-BF555) validated for, and at what dilution?

bsm-33005M-BF555 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does V5 tag Monoclonal Antibody, AbBy Fluor-555 Conjugated (bsm-33005M-BF555) react with?

Tested reactivity: Species independent.

How should bsm-33005M-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise V5 tag Monoclonal Antibody, AbBy Fluor-555 Conjugated (bsm-33005M-BF555)?

KLH conjugated V5-tag peptide

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-33005M-BF555)?

AbBy Fluor-555 on bsm-33005M-BF555: excitation / emission 553nm/568nm.