T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33012M-BF680 |
| Product Name | T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide of T7-tag:MASMTGGQQMG |
| Clonality | Monoclonal |
| Clone # | 3G7 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag. |
| Background | The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-33012M-BF680) validated for, and at what dilution?
bsm-33012M-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-33012M-BF680) react with?
Tested reactivity: Species independent.
How should bsm-33012M-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-33012M-BF680)?
KLH conjugated synthetic peptide of T7-tag:MASMTGGQQMG
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-33012M-BF680)?
AbBy Fluor-680 on bsm-33012M-BF680: excitation / emission 679nm/702nm.