Trx-Tag Monoclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33018m-biotin |
| Product Name | Trx-Tag Monoclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Human, Pig, Species independent |
| Specifications | |
| Conjugation | Biotin |
| Host | Mouse |
| Source | Recombinant human Thioredoxin |
| Clonality | Monoclonal |
| Clone # | 7G10 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7295 |
| Swiss Prot | P10599 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903; Trx-Tag. |
| Background | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is Trx-Tag Monoclonal Antibody, Biotin Conjugated (bsm-33018M-Biotin) validated for, and at what dilution?
bsm-33018M-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Trx-Tag Monoclonal Antibody, Biotin Conjugated (bsm-33018M-Biotin) react with?
Tested reactivity: Human, Pig, Species independent.
How should bsm-33018M-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Trx-Tag Monoclonal Antibody, Biotin Conjugated (bsm-33018M-Biotin)?
Recombinant human Thioredoxin