Help

beta-Actin Recombinant Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Chicken
  • Rabbit
  • Hamster
Overview
Catalog # bsm-33032r-bf555-100uL
Product Name beta-Actin Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Chicken, Rabbit, Hamster
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source Synthetic MAP peptide derived from human beta-Actin
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 60
Swiss Prot P60709
Synonyms ACTB_HUMAN; Actin, cytoplasmic 1; ACTB; EC:3.6.4.- ; Beta actin; Actin, cytoplasmic 1, N-terminally processed; actin beta; BNS; CSMH; DDS1; THC8; BKRNS; BRWS1; PS1TP5BP1; Actin cytoplasmic 1; β actin; β-actin; βactin;
Application Dilution
WB 1:20000-200000
FCM FCM1:100-500
IF(IHC-P) IFIHC-P1:200-1000

Frequently asked questions

Which applications is beta-Actin Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-33032R-BF555) validated for, and at what dilution?

bsm-33032R-BF555 is validated for WB(WB) (1:20000-200000); FCM(FCM) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does beta-Actin Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-33032R-BF555) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Chicken, Rabbit, Hamster.

How should bsm-33032R-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise beta-Actin Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-33032R-BF555)?

Synthetic MAP peptide derived from human beta-Actin

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-33032R-BF555)?

AbBy Fluor-555 on bsm-33032R-BF555: excitation / emission 553nm/568nm.