GAPDH Monoclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33033m-100uL |
| Product Name | GAPDH Monoclonal Antibody |
| Applications |
WB
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat, Hamster |
| Predicted Reactivity | Dog, Sheep, Chicken, Pig, Rabbit, Monkey |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human GAPDH |
| Clonality | Monoclonal |
| Clone # | 4F8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2597 |
| Swiss Prot | P04406 |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; CDABP0047, OK/SW-cl.12 |
| Background | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. |
| Application Dilution | |
| WB | 1:200-400 |
| IHC-P | 1:20-100 |
| FCM | 1:300-5000 |
Frequently asked questions
Which applications is GAPDH Monoclonal Antibody (bsm-33033M) validated for, and at what dilution?
bsm-33033M is validated for WB (1:200-400); IHC-P (1:20-100); FCM (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GAPDH Monoclonal Antibody (bsm-33033M) react with?
Tested reactivity: Human, Mouse, Rat, Hamster. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Chicken, Pig, Rabbit, Monkey.
How should bsm-33033M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GAPDH Monoclonal Antibody (bsm-33033M)?
KLH conjugated synthetic peptide derived from human GAPDH