GAPDH (4F8) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
  • Pig
Overview
Catalog # bsm-33033M
Product Name GAPDH (4F8) Monoclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human, Mouse, Rat, Pig
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human GAPDH
Clonality Monoclonal
Clone # 4F8
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2597
Swiss Prot P04406
Synonyms Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; CDABP0047, OK/SW-cl.12
Background Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400