beta-Actin Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33036m-cy3-100l |
| Product Name | beta-Actin Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Specificity | The immunogen of this antibody has sequence similarity to actin cytoplasmic 2 and beta-actin-like protein 2, and may bind to these targets. |
| Reactivity | Human, Mouse, Rat, Bovine, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human beta-Actin |
| Clonality | Monoclonal |
| Clone # | 1A2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 60 |
| Swiss Prot | P60709 |
| Synonyms | Actin, cytoplasmic 1; ACTB |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | |
| IF | 1:300-5000 |
Frequently asked questions
Which applications is beta-Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33036M-Cy3) validated for, and at what dilution?
bsm-33036M-Cy3 is validated for WB; IF (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta-Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33036M-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Pig.
How should bsm-33036M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta-Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33036M-Cy3)?
KLH conjugated synthetic peptide derived from human beta-Actin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-33036M-Cy3)?
Cy3 on bsm-33036M-Cy3: excitation / emission 512,550nm/570,615nm.