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beta I Tubulin Recombinant Antibody, HRP Conjugated

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta I Tubulin Monoclonal Antibody (bsm-33041R-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with beta I Tubulin monoclonal antibody, conjugated (bsm-33041R-HRP) at 1:50000 dilution and 4\u00b0C overnight incubation.
25 ug total protein per lane of various lysates (see on figure) probed with beta I Tubulin monoclonal antibody, conjugated (bsm-33041R-HRP) at 1:50000 dilution and 4\u00b0C overnight incubation.
Bioss conjugated antibody and packaging; contents and labels vary by product.
Bioss conjugated antibody and packaging; contents and labels vary by product.

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-33041r-hrp-100uL
Product Name beta I Tubulin Recombinant Antibody, HRP Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human beta I Tubulin
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 81027
Swiss Prot Q9H4B7
Synonyms 2810484G07Rik; Beta tubulin 1, class VI; Class VI beta tubulin; dJ543J19.4; M(beta)1; TBB1_HUMAN; TUBB1; Tubulin beta 1 class VI; Tubulin beta-1 chain; Tubulin, beta 1; tubulin, beta1;
Application Dilution
WB 1:50000-100000
IHC-P IHC-P1:100-500
IHC-F IHC-F1:100-500

Frequently asked questions

Which applications is beta I Tubulin Recombinant Antibody, HRP Conjugated (bsm-33041R-HRP) validated for, and at what dilution?

bsm-33041R-HRP is validated for WB(WB) (1:50000-100000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does beta I Tubulin Recombinant Antibody, HRP Conjugated (bsm-33041R-HRP) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-33041R-HRP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise beta I Tubulin Recombinant Antibody, HRP Conjugated (bsm-33041R-HRP)?

KLH conjugated synthetic peptide derived from human beta I Tubulin

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