Histone H3 Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33042r-cy5 |
| Product Name | Histone H3 Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bee, Hamster |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H3 |
| Clonality | Monoclonal |
| Clone # | 3G1 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8968 |
| Swiss Prot | Q71DI3 |
| Synonyms | H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD; |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. |
| Application Dilution | |
| WB | 1:5000-50000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:500-2000 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is Histone H3 Monoclonal Antibody, Cy5 Conjugated (bsm-33042R-Cy5) validated for, and at what dilution?
bsm-33042R-Cy5 is validated for WB(WB) (1:5000-50000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:500-2000); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 Monoclonal Antibody, Cy5 Conjugated (bsm-33042R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Bee, Hamster.
How should bsm-33042R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 Monoclonal Antibody, Cy5 Conjugated (bsm-33042R-Cy5)?
KLH conjugated synthetic peptide derived from human Histone H3
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-33042R-Cy5)?
Cy5 on bsm-33042R-Cy5: excitation / emission 625,650nm/670nm.