C-erbB-2/HER2 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33051m-100uL |
| Product Name | C-erbB-2/HER2 Monoclonal Antibody |
| Applications |
WB
IHC-F
IF(IHC-P)
IF(ICC)
IF
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human C-erbB-2/HER2 |
| Clonality | Monoclonal |
| Clone # | 13H9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2064 |
| Swiss Prot | P04626 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Erb-B2 Receptor Tyrosine Kinase 2; HER2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Receptor Tyrosine-Protein Kinase ErbB-2; C-Erb B2/Neu Protein; CD340 Antigen; Herstatin; EC 2.7.10; CD340; HER-2; MLN19; TKR1; |
| Background | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is C-erbB-2/HER2 Monoclonal Antibody (bsm-33051M) validated for, and at what dilution?
bsm-33051M is validated for WB (1:300-5000); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C-erbB-2/HER2 Monoclonal Antibody (bsm-33051M) react with?
Tested reactivity: Human.
How should bsm-33051M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C-erbB-2/HER2 Monoclonal Antibody (bsm-33051M)?
KLH conjugated synthetic peptide derived from human C-erbB-2/HER2