CD45 Monoclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33052m-apc |
| Product Name | CD45 Monoclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Mouse |
| Source | Recombinant human CD45 |
| Clonality | Monoclonal |
| Clone # | 14A9 |
| Isotype | IgG1 |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5788 |
| Swiss Prot | P08575 |
| Subcellular location | Cytomembrane |
| Synonyms | B220; CD 45; CD-45; CD45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Ly-5 glycoprotein; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency. |
| Background | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD45 Monoclonal Antibody, APC Conjugated (bsm-33052M-APC) validated for, and at what dilution?
bsm-33052M-APC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD45 Monoclonal Antibody, APC Conjugated (bsm-33052M-APC) react with?
Tested reactivity: Human.
How should bsm-33052M-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD45 Monoclonal Antibody, APC Conjugated (bsm-33052M-APC)?
Recombinant human CD45
What are the excitation and emission wavelengths of the APC conjugate (bsm-33052M-APC)?
APC on bsm-33052M-APC: excitation / emission 650nm/660nm.