Cytokeratin 8 Monoclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33061M-APC |
| Product Name | Cytokeratin 8 Monoclonal Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Mouse |
| Source | Recombinant human CK8 |
| Clonality | Monoclonal |
| Clone # | 10A8 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3856 |
| Swiss Prot | P05787 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8. |
| Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is Cytokeratin 8 Monoclonal Antibody, APC Conjugated (bsm-33061M-APC) validated for, and at what dilution?
bsm-33061M-APC is validated for IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytokeratin 8 Monoclonal Antibody, APC Conjugated (bsm-33061M-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33061M-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytokeratin 8 Monoclonal Antibody, APC Conjugated (bsm-33061M-APC)?
Recombinant human CK8
What are the excitation and emission wavelengths of the APC conjugate (bsm-33061M-APC)?
APC on bsm-33061M-APC: excitation / emission 650nm/660nm.