Ki-67 Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33070M-RBITC |
| Product Name | Ki-67 Monoclonal Antibody, RBITC Conjugated |
| Applications |
FCM
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Ki-67 |
| Clonality | Monoclonal |
| Clone # | 6B9 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4288 |
| Swiss Prot | P46013 |
| Synonyms | Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN. |
| Background | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF | |
Frequently asked questions
Which applications is Ki-67 Monoclonal Antibody, RBITC Conjugated (bsm-33070M-RBITC) validated for, and at what dilution?
bsm-33070M-RBITC is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ki-67 Monoclonal Antibody, RBITC Conjugated (bsm-33070M-RBITC) react with?
Tested reactivity: Human.
How should bsm-33070M-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ki-67 Monoclonal Antibody, RBITC Conjugated (bsm-33070M-RBITC)?
KLH conjugated synthetic peptide derived from human Ki-67
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-33070M-RBITC)?
RBITC on bsm-33070M-RBITC: excitation / emission 540nm/625nm.