Histone H3 (tri methyl K4) Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33110M-Cy3 |
| Product Name | Histone H3 (tri methyl K4) Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Others |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Histone H3 (tri methyl K4) |
| Modification Site | K4 |
| Clonality | Monoclonal |
| Clone # | 4E11 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Synonyms | Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4 ; Histone H3.1t ; HIST3H3; HGNC:4778; H31T_HUMAN |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Histone H3 (tri methyl K4) Monoclonal Antibody, Cy3 Conjugated (bsm-33110M-Cy3) validated for, and at what dilution?
bsm-33110M-Cy3 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (tri methyl K4) Monoclonal Antibody, Cy3 Conjugated (bsm-33110M-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Others.
How should bsm-33110M-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (tri methyl K4) Monoclonal Antibody, Cy3 Conjugated (bsm-33110M-Cy3)?
KLH conjugated synthetic peptide derived from human Histone H3 (tri methyl K4)
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-33110M-Cy3)?
Cy3 on bsm-33110M-Cy3: excitation / emission 512,550nm/570,615nm.