Fumarase/FH (4C12) Monoclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33167m-percp-100uL |
| Product Name | Fumarase/FH (4C12) Monoclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Mouse |
| Source | Recombinant human Fumarase/FH Protein |
| Clonality | Monoclonal |
| Clone # | 4C12 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2271 |
| Swiss Prot | P07954 |
| Subcellular location | Cytoplasm, Mitochondrion matrix |
| Synonyms | FH; Fumarase; Fumarate hydratase; Fumarate hydratase mitochondrial; FUMH_HUMAN; HLRCC; LRCC; MCL; MCUL 1; MCUL1; Multiple hereditary cutaneous leiomyomata. |
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | =1:500-3000 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is Fumarase/FH (4C12) Monoclonal Antibody, PerCP Conjugated (bsm-33167M-PerCP) validated for, and at what dilution?
bsm-33167M-PerCP is validated for WB (WB=1:500-3000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Fumarase/FH (4C12) Monoclonal Antibody, PerCP Conjugated (bsm-33167M-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33167M-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fumarase/FH (4C12) Monoclonal Antibody, PerCP Conjugated (bsm-33167M-PerCP)?
Recombinant human Fumarase/FH Protein
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-33167M-PerCP)?
PerCP on bsm-33167M-PerCP: excitation / emission 482nm/677nm.