Vimentin Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33170m-cy5 |
| Product Name | Vimentin Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Specificity | KO-Validated |
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Mouse |
| Source | Recombinant human Vimentin Protein |
| Clonality | Monoclonal |
| Clone # | 3B7 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7431 |
| Swiss Prot | P08670 |
| Synonyms | Vimentin; VIM |
| Background | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 Publication Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Vimentin Monoclonal Antibody, Cy5 Conjugated (bsm-33170M-Cy5) validated for, and at what dilution?
bsm-33170M-Cy5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vimentin Monoclonal Antibody, Cy5 Conjugated (bsm-33170M-Cy5) react with?
Tested reactivity: Human.
How should bsm-33170M-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Vimentin Monoclonal Antibody, Cy5 Conjugated (bsm-33170M-Cy5)?
Recombinant human Vimentin Protein
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-33170M-Cy5)?
Cy5 on bsm-33170M-Cy5: excitation / emission 625,650nm/670nm.