Histone H2B Monoclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33174m-percp-100ul |
| Product Name | Histone H2B Monoclonal Antibody, PerCP Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Histone H2B |
| Clonality | Monoclonal |
| Clone # | 4H9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3018 |
| Swiss Prot | P33778 |
| Subcellular location | Nucleus |
| Synonyms | H2B.1 A; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2B/l. |
| Background | In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA, making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved. |
| Application Dilution | |
| IF | =1:100-500 |
Frequently asked questions
Which applications is Histone H2B Monoclonal Antibody, PerCP Conjugated (bsm-33174M-PerCP) validated for, and at what dilution?
bsm-33174M-PerCP is validated for IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H2B Monoclonal Antibody, PerCP Conjugated (bsm-33174M-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33174M-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H2B Monoclonal Antibody, PerCP Conjugated (bsm-33174M-PerCP)?
KLH conjugated synthetic peptide derived from human Histone H2B
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-33174M-PerCP)?
PerCP on bsm-33174M-PerCP: excitation / emission 482nm/677nm.