eNOS Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33176M-100uL |
| Product Name | eNOS Monoclonal Antibody |
| Applications |
WB
IHC-F
IF(ICC)
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human eNOS Protein |
| Clonality | Monoclonal |
| Clone # | 8G1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4846 |
| Swiss Prot | P29474 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
| Background | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is eNOS Monoclonal Antibody (bsm-33176M) validated for, and at what dilution?
bsm-33176M is validated for WB (1:300-5000); IHC-F (1:100-500); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eNOS Monoclonal Antibody (bsm-33176M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33176M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eNOS Monoclonal Antibody (bsm-33176M)?
Recombinant human eNOS Protein