Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

EYFP (1H9) Monoclonal Antibody

EYFP tag was immunoprecipitated from EYFP expression vector whole cell lysate with bsm-33182M at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.\r\nLane 1: EYFP expression vector whole cell lysate (Input).\r\nLane 2: bsm-33182M IP in EYFP expression vector whole cell lysate.\r\nLane 3: native mouse IgG IP in EYFP expression vector whole cell lysate (negative control).\r\n
EYFP tag was immunoprecipitated from EYFP expression vector whole cell lysate with bsm-33182M at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.\r\nLane 1: EYFP expression vector whole cell lysate (Input).\r\nLane 2: bsm-33182M IP in EYFP expression vector whole cell lysate.\r\nLane 3: native mouse IgG IP in EYFP expression vector whole cell lysate (negative control).\r\n
2 ug Recombinant EYFP protein, His (bs-33182P) per lane probed with EYFP monoclonal antibody respectively, unconjugated (bsm-33182M) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
2 ug Recombinant EYFP protein, His (bs-33182P) per lane probed with EYFP monoclonal antibody respectively, unconjugated (bsm-33182M) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Species independent
Overview
Catalog # bsm-33182m-100l
Product Name EYFP (1H9) Monoclonal Antibody
Applications
WB
Reactivity Species independent
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant EYFP protein
Clonality Monoclonal
Clone # 1H9
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms EYFPTag; EYFP Tag; Enhanced Yellow Fluorescent Protein;
Background Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is EYFP (1H9) Monoclonal Antibody (bsm-33182M) validated for, and at what dilution?

bsm-33182M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EYFP (1H9) Monoclonal Antibody (bsm-33182M) react with?

Tested reactivity: Species independent.

How should bsm-33182M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EYFP (1H9) Monoclonal Antibody (bsm-33182M)?

Recombinant EYFP protein

Search our catalog of hundreds of thousands of products