AmCyan (5F9) Monoclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33184m-pe-cy5-100ul |
| Product Name | AmCyan (5F9) Monoclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Mouse |
| Source | Recombinant AmCyan |
| Clonality | Monoclonal |
| Clone # | 5F9 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is AmCyan (5F9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33184M-PE-Cy5) validated for, and at what dilution?
bsm-33184M-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AmCyan (5F9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33184M-PE-Cy5) react with?
Tested reactivity: Species independent.
How should bsm-33184M-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AmCyan (5F9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33184M-PE-Cy5)?
Recombinant AmCyan
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-33184M-PE-Cy5)?
PE-Cy5 on bsm-33184M-PE-Cy5: excitation / emission 488nm/670nm.