ECFP (10H5) Monoclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33189m-pe-100ul |
| Product Name | ECFP (10H5) Monoclonal Antibody, PE Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | PE |
| Host | Mouse |
| Source | Recombinant ECFP |
| Clonality | Monoclonal |
| Clone # | 10H5 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | ECFPTag; ECFPTag; tagECFP; Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein; ECFP-Tag. |
| Background | Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ECFP (10H5) Monoclonal Antibody, PE Conjugated (bsm-33189M-PE) validated for, and at what dilution?
bsm-33189M-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ECFP (10H5) Monoclonal Antibody, PE Conjugated (bsm-33189M-PE) react with?
Tested reactivity: Species independent.
How should bsm-33189M-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ECFP (10H5) Monoclonal Antibody, PE Conjugated (bsm-33189M-PE)?
Recombinant ECFP
What are the excitation and emission wavelengths of the PE conjugate (bsm-33189M-PE)?
PE on bsm-33189M-PE: excitation / emission 496,564nm/578nm.