Survivin Monoclonal Antibody

Jurkat lysates probed with Survivin Polyclonal Antibody, Unconjugated (bs-33195M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Jurkat lysates probed with Survivin Polyclonal Antibody, Unconjugated (bs-33195M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Human MOLT-4 cell lysates; Lane 2: Human U937 cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human MOLT-4 cell lysates; Lane 2: Human U937 cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human MOLT-4 cell lysates; Lane 6: Human U-2OS cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human MOLT-4 cell lysates; Lane 6: Human U-2OS cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-33195M
Product Name	Survivin Monoclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Survivin
Clonality	Monoclonal
Clone #	Mix-mA?
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	332
Swiss Prot	O15392
Subcellular location	Cytoplasm, Nucleus
Synonyms	API4; API-4; API 4; Survivin variant 3 alpha; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC-5; EPR 1; EPR-1; IAP4; IAP-4; IAP 4; SVV; TIAP; BIRC5_HUMAN.??
Background	May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Belongs to the IAP family.?
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF

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