Help

Pan Methylated Lysine Monoclonal Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Species independent
Overview
Catalog # bsm-33197m-fitc-100uL
Product Name Pan Methylated Lysine Monoclonal Antibody, FITC Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Species independent
Specifications
Conjugation FITC
Host Mouse
Source BSA conjugated Methylated Lysine (di methyl, mono methyl, Trimethyl-lysine)
Clonality Monoclonal
Clone # 7F4
Isotype IgG1
Concentration 1mg/ml
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms Dimethylysine; Methyllysine; Trimethyllysine; Dimethy-lysine; Methyl-lysine; Trimethyl-lysine; N epsilon dimethyl lysine; N epsilon mono methyllysine; N epsilon trimethyl lysine; N(epsilon) trimethyl lysine; di methyl-lysine, mono methyl-lysine, Trimethyl-lysine; methyl K pan; di methyl-K, mono methyl-K, Trimethyl-K.
Application Dilution
WB 1:1000-5000
FCM FCM1μg/Test
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:100-200

Frequently asked questions

Which applications is Pan Methylated Lysine Monoclonal Antibody, FITC Conjugated (bsm-33197M-FITC) validated for, and at what dilution?

bsm-33197M-FITC is validated for WB(WB) (1:1000-5000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Pan Methylated Lysine Monoclonal Antibody, FITC Conjugated (bsm-33197M-FITC) react with?

Tested reactivity: Human, Species independent.

How should bsm-33197M-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Pan Methylated Lysine Monoclonal Antibody, FITC Conjugated (bsm-33197M-FITC)?

BSA conjugated Methylated Lysine (di methyl, mono methyl, Trimethyl-lysine)

What are the excitation and emission wavelengths of the FITC conjugate (bsm-33197M-FITC)?

FITC on bsm-33197M-FITC: excitation / emission 494nm/518nm.