HSP27 Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33203M-FITC-100uL |
| Product Name | HSP27 Monoclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | Recombinant human HSP27 Protein |
| Clonality | Monoclonal |
| Clone # | 1G12 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3315 |
| Swiss Prot | P04792 |
| Synonyms | Heat shock protein beta-1; HSPB1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28 |
| Background | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is HSP27 Monoclonal Antibody, FITC Conjugated (bsm-33203M-FITC) validated for, and at what dilution?
bsm-33203M-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSP27 Monoclonal Antibody, FITC Conjugated (bsm-33203M-FITC) react with?
Tested reactivity: Human.
How should bsm-33203M-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSP27 Monoclonal Antibody, FITC Conjugated (bsm-33203M-FITC)?
Recombinant human HSP27 Protein
What are the excitation and emission wavelengths of the FITC conjugate (bsm-33203M-FITC)?
FITC on bsm-33203M-FITC: excitation / emission 494nm/518nm.