c-fos(8A3) Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33204M-Cy5 |
| Product Name | c-fos(8A3) Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Mouse |
| Source | Recombinant human c-fos Protein |
| Clonality | Monoclonal |
| Clone # | 8A3 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2353 |
| Swiss Prot | P01100 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogene homolog; AP-1; p55; FOS_HUMAN; Proto-oncogene c-Fos; G0/G1 switch regulatory protein 7. |
| Background | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is c-fos(8A3) Monoclonal Antibody, Cy5 Conjugated (bsm-33204M-Cy5) validated for, and at what dilution?
bsm-33204M-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does c-fos(8A3) Monoclonal Antibody, Cy5 Conjugated (bsm-33204M-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33204M-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise c-fos(8A3) Monoclonal Antibody, Cy5 Conjugated (bsm-33204M-Cy5)?
Recombinant human c-fos Protein
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-33204M-Cy5)?
Cy5 on bsm-33204M-Cy5: excitation / emission 625,650nm/670nm.