Phosphotyrosine Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33205M-FITC-100uL |
| Product Name | Phosphotyrosine Monoclonal Antibody, FITC Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | KLH conjugated Phosphotyrosine |
| Modification Site | Phosphoserine |
| Clonality | Monoclonal |
| Clone # | 4D3 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Phospho-tyrosine; Phospho tyrosine; Phospho-tyr; Phospho tyr; p-Tyr; p-tyrosine; |
| Background | The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is Phosphotyrosine Monoclonal Antibody, FITC Conjugated (bsm-33205M-FITC) validated for, and at what dilution?
bsm-33205M-FITC is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phosphotyrosine Monoclonal Antibody, FITC Conjugated (bsm-33205M-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33205M-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phosphotyrosine Monoclonal Antibody, FITC Conjugated (bsm-33205M-FITC)?
KLH conjugated Phosphotyrosine
What are the excitation and emission wavelengths of the FITC conjugate (bsm-33205M-FITC)?
FITC on bsm-33205M-FITC: excitation / emission 494nm/518nm.