TNF alpha Monoclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33207m-bf750 |
| Product Name | TNF alpha Monoclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Chicken, Rabbit, Monkey, GuineaPig, yeast |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Mouse |
| Source | Recombinant human TNF alpha 77-233aa |
| Clonality | Monoclonal |
| Clone # | 3A4 |
| Isotype | IgG1 |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7124 |
| Swiss Prot | P01375 |
| Subcellular location | Secreted, Cytomembrane |
| Synonyms | tumor necrosis factor-_; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha. |
| Background | Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TNF alpha Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33207M-BF750) validated for, and at what dilution?
bsm-33207M-BF750 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TNF alpha Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33207M-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Chicken, Rabbit, Monkey, GuineaPig, yeast.
How should bsm-33207M-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TNF alpha Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33207M-BF750)?
Recombinant human TNF alpha 77-233aa
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-33207M-BF750)?
AbBy Fluor-750 on bsm-33207M-BF750: excitation / emission 749nm/775nm.