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Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP Conjugated

Applications

  • WB

Reactivity

  • Species independent
Overview
Catalog # bsm-33215M-PerCP-100uL
Product Name Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP Conjugated
Applications
WB
Reactivity Species independent
Specifications
Conjugation PerCP
Host Mouse
Source KLH conjugated synthetic peptide of Nano-Tag (9)
Clonality Monoclonal
Clone # 9
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms Nano-Tag (9); Nano-Tag9; Nano-Tag; Nano Tag (9);
Background Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The Nano-tag is a new streptavidin-binding peptide for both the purification and the detection of Nano-tagged proteins. This peptide possesses nanomolar-affinity for streptavidin and therefore is termed Nano-tag. The nano-tags have two types, Nano-tag15 (MDVEAWLGARVPLVET) and Nano-tag9 (MDVEAWLGAR), which bind to streptavidin with dissociation constants of 4 nM and 17 nM, respectively.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP Conjugated (bsm-33215M-PerCP) validated for, and at what dilution?

bsm-33215M-PerCP is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP Conjugated (bsm-33215M-PerCP) react with?

Tested reactivity: Species independent.

How should bsm-33215M-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP Conjugated (bsm-33215M-PerCP)?

KLH conjugated synthetic peptide of Nano-Tag (9)

What are the excitation and emission wavelengths of the PerCP conjugate (bsm-33215M-PerCP)?

PerCP on bsm-33215M-PerCP: excitation / emission 482nm/677nm.