STAT3 Monoclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33218m-percp-cy55-100ul |
| Product Name | STAT3 Monoclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Mouse |
| Immunogen Range | 9-265/770 |
| Clonality | Monoclonal |
| Clone # | 3F5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6774 |
| Swiss Prot | P40763 |
| Synonyms | Signal transducer and activator of transcription 3; STAT3; Acute-phase response factor; APRF |
| Background | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genesPubMed:12359225. Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is STAT3 Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-33218M-PERCP-CY5-5) validated for, and at what dilution?
BSM-33218M-PERCP-CY5-5 is validated for WB (1:300-5000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does STAT3 Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-33218M-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-33218M-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-33218M-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-33218M-PERCP-CY5-5: excitation / emission 482nm/694nm.