STAT3 Monoclonal antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33223m-pe-cy7 |
| Product Name | STAT3 Monoclonal antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Mouse |
| Source | Recombinant human STAT3 Protein |
| Clonality | Monoclonal |
| Clone # | 5H4 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6774 |
| Swiss Prot | P40763 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3 (acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN. |
| Background | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is STAT3 Monoclonal antibody, PE-Cy7 Conjugated (bsm-33223M-PE-Cy7) validated for, and at what dilution?
bsm-33223M-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does STAT3 Monoclonal antibody, PE-Cy7 Conjugated (bsm-33223M-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33223M-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise STAT3 Monoclonal antibody, PE-Cy7 Conjugated (bsm-33223M-PE-Cy7)?
Recombinant human STAT3 Protein
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-33223M-PE-Cy7)?
PE-Cy7 on bsm-33223M-PE-Cy7: excitation / emission 488nm/780nm.