GRP78/Bip Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33224M-Cy5.5 |
| Product Name | GRP78/Bip Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human GRP78/Bip |
| Clonality | Monoclonal |
| Clone # | 10A3 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3309 |
| Swiss Prot | P11021 |
| Subcellular location | Cytoplasm |
| Synonyms | BIP; HSPA5; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN. |
| Background | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GRP78/Bip Monoclonal Antibody, Cy5.5 Conjugated (BSM-33224M-CY5-5) validated for, and at what dilution?
BSM-33224M-CY5-5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRP78/Bip Monoclonal Antibody, Cy5.5 Conjugated (BSM-33224M-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should BSM-33224M-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRP78/Bip Monoclonal Antibody, Cy5.5 Conjugated (BSM-33224M-CY5-5)?
KLH conjugated synthetic peptide derived from human GRP78/Bip
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-33224M-CY5-5)?
Cy5.5 on BSM-33224M-CY5-5: excitation / emission 675nm/694nm.