ERK1/2 Monoclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33232m-pe-cy5 |
| Product Name | ERK1/2 Monoclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human ERK1/2 |
| Clonality | Monoclonal |
| Clone # | 8C2 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5595 |
| Swiss Prot | P27361 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P44/42 MAPK; ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3. |
| Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:1000-5000 |
| FCM | FCM1μg/Test |
Frequently asked questions
Which applications is ERK1/2 Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33232M-PE-Cy5) validated for, and at what dilution?
bsm-33232M-PE-Cy5 is validated for WB(WB) (1:1000-5000); FCM(FCM) (1μg/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERK1/2 Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33232M-PE-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-33232M-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ERK1/2 Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33232M-PE-Cy5)?
KLH conjugated synthetic peptide derived from human ERK1/2
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-33232M-PE-Cy5)?
PE-Cy5 on bsm-33232M-PE-Cy5: excitation / emission 488nm/670nm.