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AMPK alpha-1 Monoclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-33236M-Biotin-100uL
Product Name AMPK alpha-1 Monoclonal Antibody, Biotin Conjugated
Applications
WB IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation Biotin
Host Mouse
Source KLH conjugated synthetic peptide derived from human AMPK alpha-1
Clonality Monoclonal
Clone # 7G11
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 5562
Swiss Prot Q13131
Synonyms AMPK alpha 1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase;hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94C10; AAPK1_HUMAN.
Background The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:5000-10000
IHC-F 1:100-500

Frequently asked questions

Which applications is AMPK alpha-1 Monoclonal Antibody, Biotin Conjugated (bsm-33236M-Biotin) validated for, and at what dilution?

bsm-33236M-Biotin is validated for WB (1:5000-10000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AMPK alpha-1 Monoclonal Antibody, Biotin Conjugated (bsm-33236M-Biotin) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-33236M-Biotin be stored?

Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AMPK alpha-1 Monoclonal Antibody, Biotin Conjugated (bsm-33236M-Biotin)?

KLH conjugated synthetic peptide derived from human AMPK alpha-1

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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