SGK1 Monoclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33275M-PE-100uL |
| Product Name | SGK1 Monoclonal Antibody, PE Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Pig, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human SGK1 |
| Clonality | Monoclonal |
| Clone # | 5E11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6446 |
| Swiss Prot | O00141 |
| Synonyms | Serum/glucocorticoid regulated kinase 1; SGK 1; SGK-1; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase; SGK 1; SGK; SGK1_HUMAN. |
| Background | SGK1 is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels of SGK1 and activity may contribute to conditions such as hypertension and diabetic nephropathy. This protein also mediates cell survival signals, as it has been shown to phosphorylate and negatively regulate the pro apoptotic FOXO3A protein. Ser 422 is a critical site on the protein and may be involved in its activation. |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is SGK1 Monoclonal Antibody, PE Conjugated (bsm-33275M-PE) validated for, and at what dilution?
bsm-33275M-PE is validated for IF(ICC) (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SGK1 Monoclonal Antibody, PE Conjugated (bsm-33275M-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Pig, Rabbit.
How should bsm-33275M-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SGK1 Monoclonal Antibody, PE Conjugated (bsm-33275M-PE)?
KLH conjugated synthetic peptide derived from human SGK1
What are the excitation and emission wavelengths of the PE conjugate (bsm-33275M-PE)?
PE on bsm-33275M-PE: excitation / emission 496,564nm/578nm.