Actin, alpha skeletal muscle Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33297m-cy3 |
| Product Name | Actin, alpha skeletal muscle Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human ACTA1 |
| Clonality | Monoclonal |
| Clone # | 3E9 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 58 |
| Swiss Prot | P68133 |
| Subcellular location | Cytoplasm |
| Synonyms | ACTS_HUMAN; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; actine; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; Beta cytoskeletal actin; nemaline myopathy type 3. |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Actin, alpha skeletal muscle Monoclonal Antibody, Cy3 Conjugated (bsm-33297M-Cy3) validated for, and at what dilution?
bsm-33297M-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Actin, alpha skeletal muscle Monoclonal Antibody, Cy3 Conjugated (bsm-33297M-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep.
How should bsm-33297M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Actin, alpha skeletal muscle Monoclonal Antibody, Cy3 Conjugated (bsm-33297M-Cy3)?
KLH conjugated synthetic peptide derived from human ACTA1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-33297M-Cy3)?
Cy3 on bsm-33297M-Cy3: excitation / emission 512,550nm/570,615nm.