phospho-STAT3 (Tyr705) Monoclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33301m-apc-Cy5.5 |
| Product Name | phospho-STAT3 (Tyr705) Monoclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Mouse |
| Source | KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705 |
| Modification Site | Tyr705 |
| Clonality | Monoclonal |
| Clone # | 3F10 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6774 |
| Swiss Prot | P40763 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | STAT3 (phospho Y705); p-STAT3 (phospho Y705); Phospho-Stat3_pTyr705? STAT3(Phospho-Tyr705); p-STAT3(Tyr705); Phosphorylated Stat3(pTyr705); p-Stat3; Acute Phase Response Factor; APRF; DNA binding protein APRF; FLJ20882; MGC16063; Signal Transductor and Activator of Transcription 3; STAT 3; STAT3_HUMAN. |
| Background | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063. |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
Frequently asked questions
Which applications is phospho-STAT3 (Tyr705) Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-33301M-APC-CY5-5) validated for, and at what dilution?
BSM-33301M-APC-CY5-5 is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-STAT3 (Tyr705) Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-33301M-APC-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, Guinea Pig.
How should BSM-33301M-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-STAT3 (Tyr705) Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-33301M-APC-CY5-5)?
KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-33301M-APC-CY5-5)?
APC-Cy5.5 on BSM-33301M-APC-CY5-5: excitation / emission 650nm/694nm.