LC3A Monoclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33309m-apc |
| Product Name | LC3A Monoclonal Antibody, APC Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human LC3A |
| Immunogen Range | 1-100/121 |
| Clonality | Monoclonal |
| Clone # | 7G10 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84557 |
| Swiss Prot | Q9H492 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A and 1B, light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN.?? |
| Background | Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is LC3A Monoclonal Antibody, APC Conjugated (bsm-33309M-APC) validated for, and at what dilution?
bsm-33309M-APC is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LC3A Monoclonal Antibody, APC Conjugated (bsm-33309M-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33309M-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LC3A Monoclonal Antibody, APC Conjugated (bsm-33309M-APC)?
KLH conjugated synthetic peptide derived from human LC3A Immunogen range: 1-100/121.
What are the excitation and emission wavelengths of the APC conjugate (bsm-33309M-APC)?
APC on bsm-33309M-APC: excitation / emission 650nm/660nm.