Gamma Tubulin Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33313m-fitc-100uL |
| Product Name | Gamma Tubulin Monoclonal Antibody, FITC Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Gamma Tubulin |
| Clonality | Monoclonal |
| Clone # | 8D11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7283 |
| Swiss Prot | P23258 |
| Synonyms | Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1. |
| Background | Centrosome Marker Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. |
| Application Dilution | |
| WB | 1:200-400 |
| IF | 1:50-100 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Gamma Tubulin Monoclonal Antibody, FITC Conjugated (bsm-33313M-FITC) validated for, and at what dilution?
bsm-33313M-FITC is validated for WB (1:200-400); IF (1:50-100); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gamma Tubulin Monoclonal Antibody, FITC Conjugated (bsm-33313M-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33313M-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gamma Tubulin Monoclonal Antibody, FITC Conjugated (bsm-33313M-FITC)?
KLH conjugated synthetic peptide derived from human Gamma Tubulin
What are the excitation and emission wavelengths of the FITC conjugate (bsm-33313M-FITC)?
FITC on bsm-33313M-FITC: excitation / emission 494nm/518nm.