Beclin 1 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33323m-100uL |
| Product Name | Beclin 1 Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Beclin 1 |
| Clonality | Monoclonal |
| Clone # | 7B12 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 8678 |
| Swiss Prot | Q14457 |
| Synonyms | Beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Background | Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. |
| Application Dilution | |
| WB | 1:100-500 |
| IF(IHC-P) | 1:100-500 |
| IHC-P | 1:100-500 |
Frequently asked questions
Which applications is Beclin 1 Monoclonal Antibody (bsm-33323M) validated for, and at what dilution?
bsm-33323M is validated for WB (1:100-500); IF(IHC-P) (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Beclin 1 Monoclonal Antibody (bsm-33323M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33323M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Beclin 1 Monoclonal Antibody (bsm-33323M)?
KLH conjugated synthetic peptide derived from human Beclin 1