HP1 gamma Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33375M-FITC-100uL |
| Product Name | HP1 gamma Monoclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | Recombinant human HP1 gamma Protein |
| Clonality | Monoclonal |
| Clone # | 5B9 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11335 |
| Swiss Prot | Q13185 |
| Subcellular location | Nucleus |
| Synonyms | CBX 3; CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma homolog; HP1Hs gamma ; Modifier 2; protein HP1-GAMMA; HP1g; M32; MGC118084; CBX3_HUMAN; Modifier 2 protein; |
| Background | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HP1 gamma Monoclonal Antibody, FITC Conjugated (bsm-33375M-FITC) validated for, and at what dilution?
bsm-33375M-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HP1 gamma Monoclonal Antibody, FITC Conjugated (bsm-33375M-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33375M-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HP1 gamma Monoclonal Antibody, FITC Conjugated (bsm-33375M-FITC)?
Recombinant human HP1 gamma Protein
What are the excitation and emission wavelengths of the FITC conjugate (bsm-33375M-FITC)?
FITC on bsm-33375M-FITC: excitation / emission 494nm/518nm.