ATG5/APG5L Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bsm-33386M) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-mouse IgG antibody (bs-0296G-AF488) for 90 minutes, and DAPI for nuclei staining.

$Lane 1: Human PANC-1 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ATG5\/APG5L Monoclonal Antibody, Unconjugated (bsm-33386M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human PANC-1 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ATG5\/APG5L Monoclonal Antibody, Unconjugated (bsm-33386M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33386m
Product Name	ATG5/APG5L Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ATG5 Protein
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9474
Swiss Prot	Q9H1Y0
Subcellular location	Cytoplasm
Synonyms	ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500