Collagen I Monoclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33400M-BF647-100uL |
| Product Name | Collagen I Monoclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Collagen I |
| Clonality | Monoclonal |
| Clone # | 7C7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1277 |
| Swiss Prot | P02452 |
| Subcellular location | Secreted, Extracellular domain |
| Synonyms | Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein. |
| Background | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]. |
| Application Dilution | |
| WB | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is Collagen I Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33400M-BF647) validated for, and at what dilution?
bsm-33400M-BF647 is validated for WB (1:100-500); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen I Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33400M-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33400M-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen I Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33400M-BF647)?
KLH conjugated synthetic peptide derived from human Collagen I
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-33400M-BF647)?
AbBy Fluor-647 on bsm-33400M-BF647: excitation / emission 650nm/665nm.