Bcl-2 Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33411M-Cy5.5 |
| Product Name | Bcl-2 Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Bcl-2 |
| Clonality | Monoclonal |
| Clone # | 3F12 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 596 |
| Swiss Prot | P10415 |
| Synonyms | Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN. |
| Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | ICC1:50-200 |
Frequently asked questions
Which applications is Bcl-2 Monoclonal Antibody, Cy5.5 Conjugated (BSM-33411M-CY5-5) validated for, and at what dilution?
BSM-33411M-CY5-5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 Monoclonal Antibody, Cy5.5 Conjugated (BSM-33411M-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-33411M-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 Monoclonal Antibody, Cy5.5 Conjugated (BSM-33411M-CY5-5)?
KLH conjugated synthetic peptide derived from human Bcl-2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-33411M-CY5-5)?
Cy5.5 on BSM-33411M-CY5-5: excitation / emission 675nm/694nm.