Claudin 2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human gall bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Claudin 2 Monoclonal Antibody, Unconjugated (bsm-33414M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human gall bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Claudin 2 Monoclonal Antibody, Unconjugated (bsm-33414M) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-33414M
Product Name	Claudin 2 Monoclonal Antibody
Applications	IHC-P IHC-F IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Claudin 2
Clonality	Monoclonal
Clone #	10G2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9075
Swiss Prot	P57739
Subcellular location	Cell membrane
Synonyms	Claudin-2; Claudin2; CLD2_HUMAN; CLDN 2; CLDN2; OTTHUMP00000023793; SP 82; SP82.
Background	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Application Dilution
IHC-P	1:200-400
IHC-F
IF