IKB alpha Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33441m-cy5 |
| Product Name | IKB alpha Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Mouse |
| Source | Recombinant human IKB alpha Protein |
| Immunogen Range | 1-77/314 |
| Clonality | Monoclonal |
| Clone # | 2A5 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P25963 |
| Swiss Prot | 4792 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN. |
| Background | Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is IKB alpha Monoclonal Antibody, Cy5 Conjugated (bsm-33441M-Cy5) validated for, and at what dilution?
bsm-33441M-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IKB alpha Monoclonal Antibody, Cy5 Conjugated (bsm-33441M-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33441M-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IKB alpha Monoclonal Antibody, Cy5 Conjugated (bsm-33441M-Cy5)?
Recombinant human IKB alpha Protein Immunogen range: 1-77/314.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-33441M-Cy5)?
Cy5 on bsm-33441M-Cy5: excitation / emission 625,650nm/670nm.