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TurboGFP (3A6) Monoclonal Antibody

Applications

  • WB
Overview
Catalog # bsm-33867M
Product Name TurboGFP (3A6) Monoclonal Antibody
Applications
WB
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant TurboGFP protein expressed in HEK293T cell
Clonality Monoclonal
Clone # 3A6
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Background TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20 % sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP.

Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is TurboGFP (3A6) Monoclonal Antibody (bsm-33867M) validated for, and at what dilution?

bsm-33867M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-33867M be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TurboGFP (3A6) Monoclonal Antibody (bsm-33867M)?

Recombinant TurboGFP protein expressed in HEK293T cell