TurboGFP (3A6) Monoclonal Antibody

$Lane 1\u30012: Recombinant TurboGFP protein expressed in HEK293T cell lysates probed with TurboGFP Monoclonal Antibody, Unconjugated (bsm-33867M) at 1:50000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1\u30012: Recombinant TurboGFP protein expressed in HEK293T cell lysates probed with TurboGFP Monoclonal Antibody, Unconjugated (bsm-33867M) at 1:50000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Overview
Catalog #	bsm-33867M
Product Name	TurboGFP (3A6) Monoclonal Antibody
Applications	WB
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant TurboGFP protein expressed in HEK293T cell
Clonality	Monoclonal
Clone #	3A6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Background	TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20 % sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP.
Application Dilution
WB	1:300-5000

Applications

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