TurboGFP (3A6) Monoclonal Antibody, Cy7 Conjugated
Applications
| Overview | |
| Catalog # | bsm-33867M-Cy7 |
| Product Name | TurboGFP (3A6) Monoclonal Antibody, Cy7 Conjugated |
| Applications |
WB
|
| Specifications | |
| Conjugation | Cy7 |
| Host | Mouse |
| Source | Recombinant TurboGFP protein expressed in HEK293T cell |
| Clonality | Monoclonal |
| Clone # | 3A6 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20 % sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TurboGFP (3A6) Monoclonal Antibody, Cy7 Conjugated (bsm-33867M-Cy7) validated for, and at what dilution?
bsm-33867M-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-33867M-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TurboGFP (3A6) Monoclonal Antibody, Cy7 Conjugated (bsm-33867M-Cy7)?
Recombinant TurboGFP protein expressed in HEK293T cell
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-33867M-Cy7)?
Cy7 on bsm-33867M-Cy7: excitation / emission 743nm/767nm.