LMNA | Lamin A/C Monoclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-34042m-apc-100uL |
| Product Name | LMNA | Lamin A/C Monoclonal Antibody, APC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Pig |
| Specifications | |
| Conjugation | APC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human lamin A |
| Clonality | Monoclonal |
| Clone # | 3.00E+01 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4000 |
| Swiss Prot | P02545 |
| Subcellular location | Nucleus |
| Synonyms | lamin-A; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; Dhe; LMNA_CHICK; LMNA; LMNA_HUMAN; LMNA_MOUSE; LMNA_PIG; LMNA_RAT; lamin A/C; cardiomyopathy, dilated 1A (autosomal dominant); limb girdle muscular dystrophy 1B (autosomal dominant); progeria 1 (Hutchinson-Gilford type); lamin A/C-like 1; mandibuloacral dysplasia type A; progerin |
| Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012] |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is LMNA | Lamin A/C Monoclonal Antibody, APC Conjugated (bsm-34042M-APC) validated for, and at what dilution?
bsm-34042M-APC is validated for WB (1:1000-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LMNA | Lamin A/C Monoclonal Antibody, APC Conjugated (bsm-34042M-APC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Pig.
How should bsm-34042M-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LMNA | Lamin A/C Monoclonal Antibody, APC Conjugated (bsm-34042M-APC)?
KLH conjugated synthetic peptide derived from human lamin A
What are the excitation and emission wavelengths of the APC conjugate (bsm-34042M-APC)?
APC on bsm-34042M-APC: excitation / emission 650nm/660nm.