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DNA-RNA Hybrid Monoclonal Antibody

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNA-RNA Hybrid) monoclonal Antibody, Unconjugated (bsm-34118M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNA-RNA Hybrid) monoclonal Antibody, Unconjugated (bsm-34118M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA-RNA Hybrid Monoclonal Antibody, Unconjugated (bsm-34118M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Species independent
Overview
Catalog # bsm-34118m
Product Name DNA-RNA Hybrid Monoclonal Antibody
Applications
ELISA IHC-P IHC-F IF ICC/IF
Reactivity Species independent
Specifications
Conjugation Unconjugated
Host Mouse
Source ΦX174 bacteriophage-derived synthetic DNA/RNA
Clonality Monoclonal
Clone # S9.6
Isotype IgG2a
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer PBS with 0.02% Proclin300 and 50% Glycerol, pH7.4.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms DNA-RNA hybrid; RNA/DNA hybrid; DNA-RNA Hybrid [S9.6];
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF
ICC/IF 1:50-100

Frequently asked questions

Which applications is DNA-RNA Hybrid Monoclonal Antibody (bsm-34118M) validated for, and at what dilution?

bsm-34118M is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DNA-RNA Hybrid Monoclonal Antibody (bsm-34118M) react with?

Tested reactivity: Species independent.

How should bsm-34118M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS with 0.02% Proclin300 and 50% Glycerol, pH7.4.

What immunogen was used to raise DNA-RNA Hybrid Monoclonal Antibody (bsm-34118M)?

ΦX174 bacteriophage-derived synthetic DNA/RNA

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