Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

PMS2 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMS2) Monoclonal Antibody, Unconjugated (bsm-34140M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMS2) Monoclonal Antibody, Unconjugated (bsm-34140M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMS2) Monoclonal Antibody, Unconjugated (bsm-34140M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMS2) Monoclonal Antibody, Unconjugated (bsm-34140M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IHC-P

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-34140m
Product Name PMS2 Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IHC-P
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human PMS2
Clonality Monoclonal
Clone # 7A11
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5395
Swiss Prot P54278
Subcellular location Nucleus
Synonyms DNA mismatch repair protein PMS2; Mismatch repair endonuclease PMS2; Mismatch repair gene PMSL2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS2_HUMAN; Postmeiotic segregation increased, S. cerevisiae, 2; DNA mismatch repair gene homologue; H_DJ0042M02.9; HNPCC4; PMS 2; PMS1 protein homolog 2; PMS2 postmeiotic segregation increased 2; PMS2CL; PMSL2.
Application Dilution
IHC-F 1:100-500
IF(IHC-P) IFIHC-P1:100-500
IHC-P IHC-P1:100-500

Frequently asked questions

Which applications is PMS2 Monoclonal Antibody (bsm-34140M) validated for, and at what dilution?

bsm-34140M is validated for IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PMS2 Monoclonal Antibody (bsm-34140M) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-34140M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PMS2 Monoclonal Antibody (bsm-34140M)?

KLH conjugated synthetic peptide derived from human PMS2

Search our catalog of hundreds of thousands of products