OCT2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (Human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (\r\nOCT2) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-34142m
Product Name	OCT2 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human OCT2
Clonality	Monoclonal
Clone #	13C4
Isotype	IgG2b, Kappa
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5452
Swiss Prot	P09086
Subcellular location	Cytoplasm, Nucleus
Synonyms	organic cation transporter OCT2; POU domain, class 2, transcription factor 2; Octamer-binding transcription factor 2; Oct-2; OTF-2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; POU2F2; NF A2; Oct 2; PO2F2_MOUSE; PO2F2_HUMAN.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF